control mab295 Search Results


osm  (R&D Systems)
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R&D Systems osm
Figure 6 In vitro exposition of reconstructed human epidermis (RHE) to <t>OSM</t> and IL-1. RHE was exposed to PBS, <t>OSM,</t> <t>IL-1b,</t> or a combination of both OSM and IL-1b (10 ng/mL each), and then fixed and stained with H&E or for cytokeratin-10. Shown are representative images of three experiments.
Osm, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6 In vitro exposition of reconstructed human epidermis (RHE) to OSM and IL-1. RHE was exposed to PBS, OSM, IL-1b, or a combination of both OSM and IL-1b (10 ng/mL each), and then fixed and stained with H&E or for cytokeratin-10. Shown are representative images of three experiments.

Journal: The American journal of pathology

Article Title: Involvement of IL-1 and oncostatin M in acanthosis associated with hypertensive leg ulcer.

doi: 10.1016/j.ajpath.2012.11.030

Figure Lengend Snippet: Figure 6 In vitro exposition of reconstructed human epidermis (RHE) to OSM and IL-1. RHE was exposed to PBS, OSM, IL-1b, or a combination of both OSM and IL-1b (10 ng/mL each), and then fixed and stained with H&E or for cytokeratin-10. Shown are representative images of three experiments.

Article Snippet: Block serial sections of 3 mmwere cut, deparaffinized in xylene, and hydrated in a graded series of alcohol.After antigen retrieval in citrate buffer, staining was performed using the BenchMark automated staining system (VentanaMedical System, Tucson, AZ) for Ki-67 (IgG1, MIB-1; Dako Cytomation, Glostrup, Denmark), a-SMA (IgG2a, clone 1A4; Dako Cytomation), cytokeratin 10 ( IgG1, SC-23877; Santa Cruz Biotechnology, Santa Cruz, CA), IL-1b (rabbit polyclonal, sc-7884; Santa Cruz Biotechnology), and OSM (IgG2a, MAB295; R&D Systems).

Techniques: In Vitro, Staining

Figure 7 Mice ear injection of IL-1b and OSM recapitulates some features of HLU. Earsfrom outbred C57B6 mice (n Z 5 for each group) were injected intradermally with 250 ng of IL-1b, 250 ng of OSM, combination of both, or with PBS as a control. On day 3, ears were collected for staining with H&E, immu- nodetection of neutrophils using antieGr-1 mAb and PCNA mAb staining. Scale bar Z 100 mm

Journal: The American journal of pathology

Article Title: Involvement of IL-1 and oncostatin M in acanthosis associated with hypertensive leg ulcer.

doi: 10.1016/j.ajpath.2012.11.030

Figure Lengend Snippet: Figure 7 Mice ear injection of IL-1b and OSM recapitulates some features of HLU. Earsfrom outbred C57B6 mice (n Z 5 for each group) were injected intradermally with 250 ng of IL-1b, 250 ng of OSM, combination of both, or with PBS as a control. On day 3, ears were collected for staining with H&E, immu- nodetection of neutrophils using antieGr-1 mAb and PCNA mAb staining. Scale bar Z 100 mm

Article Snippet: Block serial sections of 3 mmwere cut, deparaffinized in xylene, and hydrated in a graded series of alcohol.After antigen retrieval in citrate buffer, staining was performed using the BenchMark automated staining system (VentanaMedical System, Tucson, AZ) for Ki-67 (IgG1, MIB-1; Dako Cytomation, Glostrup, Denmark), a-SMA (IgG2a, clone 1A4; Dako Cytomation), cytokeratin 10 ( IgG1, SC-23877; Santa Cruz Biotechnology, Santa Cruz, CA), IL-1b (rabbit polyclonal, sc-7884; Santa Cruz Biotechnology), and OSM (IgG2a, MAB295; R&D Systems).

Techniques: Injection, Control, Staining